Concanavalin A Binding Glycoproteins of Epidermal Cells
نویسندگان
چکیده
منابع مشابه
BINDING SITES ON CONCANAVALIN A 399 Concanavalin
Equilibrium dialysis of concanavalin A against methyl c¢-D-mannopyranoside and methyl C¢-D-glucopyranoside conducted at 2 ° in the presence of I M NaC1 showed that concanavalin A is bivalent. A SCATCHARD plot of the data obtained gave straight lines for both sugars with observed association constants (K') of 1. 4. lO 4 1/mole for methyl ,¢-D-mannopyranoside and o.3" IO 4 1/mole for methyl ~¢-D-...
متن کاملComputational analysis of Concanavalin A binding glycoproteins of human seminal plasma
Glycoproteins have immense clinical importance and comparative glycoproteomics has become a powerful tool for biomarker discovery and disease diagnosis. Seminal plasma glycoproteins participate in fertility related processes including sperm-egg recognition, modulation of capacitation and acrosome reaction inhibition. Affinity chromatography using broad specificity lectin such as Con A is widely...
متن کاملProfiling of concanavalin A-binding glycoproteins in human hepatic stellate cells activated with transforming growth factor-β1.
Glycoproteins play important roles in maintaining normal cell functions depending on their glycosylations. Our previous study indicated that the abundance of glycoproteins recognized by concanavalin A (ConA) was increased in human hepatic stellate cells (HSCs) following activation by transforming growth factor-β1 (TGF-β1); however, little is known about the ConA-binding glycoproteins (CBGs) of ...
متن کاملCholesterol microcrystals associated with concanavalin A-binding glycoproteins contribute artifactually to nucleating activity assays.
Concanavalin A (Con A) affinity chromatography is the standard procedure to separate cholesterol nucleating biliary proteins from lipids and pigment. We observed that even after extensive washing following application of bile, lipid contaminants remain. We have determined the contribution of lipid contamination to cholesterol nucleation and assessed a modified procedure to remove lipids from th...
متن کاملConcanavalin A-captured glycoproteins in healthy human urine.
Both the urinary proteome and its glycoproteome can reflect human health status, and more directly, functions of kidney and urinary tracts. Because the high abundance protein albumin is not N-glycosylated, the urine N-glycoprotein enrichment procedure could deplete it, and urine proteome could thus provide a more detailed protein profile in addition to glycosylation information especially when ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Investigative Dermatology
سال: 1982
ISSN: 0022-202X
DOI: 10.1111/1523-1747.ep12500055